Eukaryotic protein expression

jstrassw at OPAL.TUFTS.EDU jstrassw at OPAL.TUFTS.EDU
Wed Aug 21 13:19:52 EST 1996


Hello!
We've had lots of luck lately expressing things in yeast.   We use 
Galactose induction.   The only problem is getting it out of yeast, but 
at least we often get full-length soluble proteins (including GST 
fusions) which are trashed in E. Cole strains.

Good Luck!
John


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John Strasswimmer, MD,PhD Candidate    | Phone (617) 636 8396
Tufts - New England Medical Center     | Fax   (617) 636 6190
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On Tue, 20 Aug 1996, Katie Walstrom wrote:

> I would like to overexpress a eukaryotic protein that gets rapidly 
> degraded when it's induced in E. coli.  It can be overexpressed in a 
> bacculovirus system, but I have heard that insect cells are expensive 
> to maintain.  Is there any other overexpression system besides 
> bacculovirus that I could try?  (yeast?)
> 
> I'd appreciate any suggestions.
> 
> Katie Walstrom
> walstrom at molbio.uoregon.edu
> 
> 



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