DNA extraction from BAC clone

Michael J. Prigge prigge at darkwing.uoregon.edu
Wed Aug 21 20:31:22 EST 1996


In article <4vg9ug$1h5 at mail.TJU.EDU>, Hiroshi Inoue
<hinoue at lac.jci.tju.edu> wrote:

> Hi Netter!
> 
> Does anyone know a good way to extract DNA from large-prepped BAC?
> I usually use Qiagen MIDI or MAXI kit (500ml to 1000ml culture media), 
> however, the final amount was under 20ug.
> I need 100ug or more.
> Any suggestion will be appreciated!!!
> 
> 
> Hiroshi Inoue

For my BAC preps, I simply done plain alk lysis preps. I am averaging ~1
ug/mL of culture. I use "Turbo Broth" to get high cell density, but this
probably doesn't make much difference. My preps are plenty pure enough for
my uses so far. 
mike

Michael J. Prigge                     prigge at darkwing.uoregon.edu
University of Oregon               Institute of Molecular Biology
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