I Need Advice on single cell differential display

Paul C. Grimm MD grimm at cc.UManitoba.CA
Wed Aug 21 07:27:21 EST 1996


Douglas B. Tully wrote:
> 
> I'd like to identify differentially expressed genes from single cells.
> 
> Can one make a cDNA library from a single cell?  Can one do
> differential display on a single cell?
> 
> Any advice would be appreciated.
> 
> Thanks in advance.
> 
> Doug Tully, NIEHS PH 919-541-2129

Check out Miyashiro Dichter and Eberwine
PNAS  1994;91:10800-10804
These people did differential display on portions of individual 
neurons...ie neurite vs axon vs cell body
-- 
Paul C. Grimm MD
AE102-820 Sherbrook
Winnipeg Man, Canada
R3A 1S1

"Practise random acts of kindness"



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