What really indicates induction?
patrics at okstate.edu
Wed Aug 21 16:42:24 EST 1996
I need advice from journal reviewers and/or authors who are knowledgible
about experimental design requirements necessary to show mRNA induction.
I will be performing northerns to quantitate mRNA. I want to show
conclusively if a particular gene (marker for differentiation) is or is
not induced by a drug treatment. I am particularly concerned about
internal controls. (In my experiment, F9 cells will be differentiating,
and I think some internal controls, such as cytoskeleton components, may
Any advice would be appreciated. Also, if you know of a paper which
demonstrates a thorough experimental design, let me know. Many papers do
not show or discuss all the controls.
Email: patrics at okstate.edu
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