A problem in topology

Rafael Maldonado rafael at howard.med.utah.edu
Sat Aug 10 19:08:16 EST 1996

On Fri, 9 Aug 1996, Ed Beaty wrote:

> The catch is, I can't use a phagemid vector to directly make ss circular 
> DNA in this case.  If anyone knows a good way to unlink two linked circles 
> (using a topoisomerase, maybe?) I'd be extremely thankful to hear it.

Yes, you may use topoisomerase I, sold by several companies (as Promega, 
not relation with the company). It is used to improve in vitro 
transcription, for example. I guess it will solve your problem, never 
tried though.


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