Discrepancy between OD-value on spec and DNA on agarose gel

[Rafael Maldonado]

On Sat, 10 Aug 1996, Botma Visser wrote:
> When I determined the 
> concentration of the plasmid DNA at 260 nm, I got a very 
> high reading, with the concentration going up to 1.1 mg/mL 
> from a 100 mL prep.  I then ran 500 ng plasmid DNA 
> (calculated using the determined concentration) on an 
> agarose gel, and saw nothing.  

Botma,

Single nucleotides also absorves at 260. I bet you have a large degradation 
in your samples. Use other way to extract plasmid.


Rafa


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