Discrepancy between OD-value on spec and DNA on agarose gel
rafael at howard.med.utah.edu
Sat Aug 10 19:11:44 EST 1996
On Sat, 10 Aug 1996, Botma Visser wrote:
> When I determined the
> concentration of the plasmid DNA at 260 nm, I got a very
> high reading, with the concentration going up to 1.1 mg/mL
> from a 100 mL prep. I then ran 500 ng plasmid DNA
> (calculated using the determined concentration) on an
> agarose gel, and saw nothing.
Single nucleotides also absorves at 260. I bet you have a large degradation
in your samples. Use other way to extract plasmid.
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