expressed protein is 5-6kda bigger than
Wulf Dirk Leuschner
wdl at gen.mpib-tuebingen.mpg.de
Thu Aug 22 14:51:32 EST 1996
imazhq at leonis.nus.sg (Zheng Huanquan) wrote:
>Does anyone have such experience that expressed protein is 5-6kda bigger
>that native one? my protein is 33kda and has additional 13aa + 6x His,
>thus the expressed protein should 35-36kda, but I only got 40-42kda
>expressed. Any explain?
it depends in what kind of organism you have the protein expressed. If
it is, e.g., in mammalian cells, glycosylation might be the problem.
Sometimes proteins have 'abnormal' mobilities in SDS-PAGE (although
their molecular weight is correct) due to some conformation (even
with SDS!) which influences the migration properties of the protein.
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