Mung bean nuclease ?

[Edward Wang]

Fourie Joubert (fourie at crimson) wrote:
: Hi
: 
: Could someone help me out with the reaction conditions for Mung bean
: nuclease? I need to convert a NcoI digested site to blunt ends, but I
: seem to be losing most of my plasmid...
: 
: Some info on reaction times and units of enzyme / ug of DNA would
: be very helpful!
: 
: Thanks in advance...
: 
: --------------
: Fourie Joubert
: Department of Biochemistry
: University of Pretoria
: fourie at scientia.up.ac.za
: http://suntiger.ee.up.ac.za/biodocs/home.html
: +27-12-420-2011


I can't remember the units I use, but I incubate @ 30 deg. for 35 min, and
then 95 deg. for 10 min to incactivate.
-- 
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Edward H. Wang                 *
Staff Resch. Assc.             *
Dept. Internal Med. -Nephrology*
UC Davis                       *
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