Factor Xa Cleavage

G E Martin G.E.Martin at shef.ac.uk
Wed Aug 14 06:22:25 EST 1996


I planned to use Factor Xa protease to cleave one of my fusion proteins 
but initial results suggest that the protease has a preference for a 
non specific site in the fusion (cuts in hours) and very little for the 
engineered site (days). Any hints/ideas/suggestions for overcoming this 
would be very gratefully received.

Thanks in advance

Giles 




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