Ligating small insert

Minh Q. Nguyen mqn1 at ea.oac.uci.edu
Tue Aug 20 17:09:04 EST 1996


Hi,

Has anyone tried ligating a very small insert (~50 bp) to your typical 
vector (e.g. Bluescript)?  If so, what is the optimal ratio of insert 
to vector and how efficient is the ligation?  Any suggestion would be 
appreciated.  Thanks.

mqn



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