Annealing of two complementary circular SS DNAs

Joe Boutell boutell at cf.ac.uk
Tue Aug 20 06:14:42 EST 1996


Hiya,
I have often used a colony prep protocol which involves boiling an E.coli 
colony picked into a small volume of TE for 10 minutes. This lyses the 
bugs, and i presume it would denature the plasmids released as well. 
However i then went on to transform some of this colony prep back into 
E.coli, and this worked fine (can't remember the numbers, but it was a 
lot). All i had done with the colony preps after heating was pop them in 
an ice bucket, so i think it is just a case of heating up and cooling 
down, and they will anneal.

Hope this helps,

Joe Boutell.




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