DNA dye for fluorometric quantitation
alex at alfred.med.monash.edu.au
alex at alfred.med.monash.edu.au
Mon Aug 19 19:00:13 EST 1996
We have found that Promega's "FluorKit DFA Fragment Analysis Core Kit v1.01"
enabled quantitation of our PCR products over a greater range than Ethidium
Bromide.
However, Promega has apparently stopped producing the kit.
Could anyone recommend an alternative dye for fluorometric DNA quantitation in
agarose gels. We are using non-fluorescently labelled primers and the
Molecular Dynamics FluorImager for gel scanning and quantitation.
Also, if anyone out there knows of a source of Promega's DFA-1Kit, we would be
greatful and more than willing to buy.
Looking forward to hearing from you,
Alex Tzanidis
Dept Medicine
Monash University
Melbourne, Australia
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