Antibodies to 6-His Tag
Michael W. Thompson
mthom0 at pop.uky.edu
Mon Aug 19 11:42:17 EST 1996
A recent article published described the synthesis of a reagent that could be used to
detect (His)6-tagged proteins on nitrocellulose blots. The reference is:
McMahan SA and Burgess RR. Single-step synthesis and characterization of biotinylated
nitriloacetic acid, a unique reagent for the detection of histidine-tagged proteins
immobilized on nitrocellulose. (1996) Anal Biochem 236, 101-106.
Good luck and let me know how it goes.
Dept. of Biochemistry
University of Kentucky
Lexington, KY 40536-0084
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