Antibodies to 6-His Tag

[Michael W. Thompson]

					A recent article published described the synthesis of a reagent that could be used to 
detect (His)6-tagged proteins on nitrocellulose blots.  The reference is:

McMahan SA and Burgess RR.  Single-step synthesis and characterization of biotinylated 
nitriloacetic acid, a unique reagent for the detection of histidine-tagged proteins 
immobilized on nitrocellulose.  (1996)  Anal Biochem 236, 101-106.

         Good luck and let me know how it goes.

Michael Thompson
Dept. of Biochemistry
University of Kentucky
Lexington, KY 40536-0084