RNA Purification

[Michael Kertesz]

MCCABE AJ wrote:
> 
> Can anyone tell me a method for efficient removal of contaminating
> DNA from RNA for use in RT-PCR. The RNA is of procaryotic origin.
> Many thanks.
> T.

Use RNAse-free DNase (several companies sell it), and either heat-kill it 
afterwards, or inactivate it by acidic phenolization.

regards,
 

Michael Kertesz

ETH-Microbiology,
ETH-Zentrum/LFV,
CH-8092 Zurich, Switzerland

tel: +41-1-632 3357
fax: +41-1-632 1148
e-mail: kertesz at micro.biol.ethz.ch