Mark.Prescott at med.monash.edu.au
Sun Aug 18 23:20:04 EST 1996
I am thinking of trying the Ni-NTA alkaline phosphatase conjugate
marketed by Quiagen for the detection of HISx6. I expect that this
product is OK for detecting proteins overexpressed in E. coli but has
anybody attempted to use it in a more demanding situation (eg. for the
detection of proteins not over expressed) in yeast or mammalian cells
with good results.
Thanks for any feedback.
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