Detecting HISx6

Mark Prescott Mark.Prescott at
Sun Aug 18 23:20:04 EST 1996

I am thinking of trying the Ni-NTA alkaline phosphatase conjugate 
marketed by Quiagen for the detection of HISx6. I expect that this 
product is OK for detecting proteins overexpressed in E. coli but has 
anybody attempted to use it in a more demanding situation (eg. for the 
detection of proteins not over expressed) in yeast or mammalian cells 
with good results.

Thanks for any feedback.

More information about the Methods mailing list