Detecting HISx6

Mark Prescott Mark.Prescott at med.monash.edu.au
Sun Aug 18 23:20:04 EST 1996


I am thinking of trying the Ni-NTA alkaline phosphatase conjugate 
marketed by Quiagen for the detection of HISx6. I expect that this 
product is OK for detecting proteins overexpressed in E. coli but has 
anybody attempted to use it in a more demanding situation (eg. for the 
detection of proteins not over expressed) in yeast or mammalian cells 
with good results.

Thanks for any feedback.
Mark 
 




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