Detecting HISx6

[Mark Prescott]

I am thinking of trying the Ni-NTA alkaline phosphatase conjugate 
marketed by Quiagen for the detection of HISx6. I expect that this 
product is OK for detecting proteins overexpressed in E. coli but has 
anybody attempted to use it in a more demanding situation (eg. for the 
detection of proteins not over expressed) in yeast or mammalian cells 
with good results.

Thanks for any feedback.
Mark