David B. Knaebel
knaebel at draco.clarkson.edu
Fri Aug 16 19:13:14 EST 1996
I and a student of mine are using E coli C600 for some cloning and want
to transform it with two plasmids, one a pUC based vector and one a
pACYC184 based replicon. The transformants pick up the phenotype of the
plasmid markers (amp and tet (in the case of the modified pACYC184)).
However, after several different rounds and methods of DNA isolation
followed by appropriate diagnostic restriction digests, our results are
equivocal. Has anyone had odd results like this with C600? If so, did you
find/develop any methods that gave appropriate results? We have done the
same experiments in DH10 (BRL) and have normal results.
Thanks for any help.
More information about the Methods