Westerns-blotting-BSA

Steve smiles at bgsm.edu
Thu Aug 22 13:13:29 EST 1996


Hey all!

I'm doing a normal western blot, but our polyclonal antibodies seem to be 
binding to many of the E. Coli proteins. It is possible that the 
antibodies were raised to our protein, as well as any bacterial proteins 
present in the blood during an infection in the rabbit.

Is there any protocol to add cell extract to the BSA blocking buffer, or 
the primary antibodies, which will prevent all this binding?

Or is there a better way to block?

Thanks.

STEVE



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