expressed protein is 5-6kda bigger than

[Zheng Huanquan]

: Christoph H. Winter (chw at sirius.mgen.uni-heidelberg.de) wrote:

: > The Qiaexpressionist the manual given with the Qiagen IMAC method,
: > describes the effect that proteins with a his-tag often run slightly
: > slower than they should (by calculation).
: > 
: > In our workgroup this was found as well!

Can you please post some results in your workshop here? any reason?

Thanks!

H Zheng