DNA extraction from BAC clone

Hiroshi Inoue hinoue at lac.jci.tju.edu
Wed Aug 21 19:34:24 EST 1996


Hi Netter!

Does anyone know a good way to extract DNA from large-prepped BAC?
I usually use Qiagen MIDI or MAXI kit (500ml to 1000ml culture media), 
however, the final amount was under 20ug.
I need 100ug or more.
Any suggestion will be appreciated!!!


Hiroshi Inoue

Kimmel Cancer Institute
Thosmas Jefferson University
Philadelphia





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