Some polymerase better for PCR?

Nick Jacobsen jacobsen at cf.ac.uk
Fri Aug 16 07:53:17 EST 1996


Hi there,
        I have used different thermostable polymerases for difficult 
PCRs. I have found that New England Biolabs Deep Vent exo- polymerase was 
excellent. NEB do not advocate this enzymes use in PCR. It does however 
do a good job. You can denature your DNA at 98 C without the enzyme 
dying.

hope this helps

cheers 

Nick.




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