Problems with ligation after GeneCleaning

Anis Limami limami at versailles.inra.fr
Thu Aug 22 06:33:39 EST 1996


I suffered with the same probleme as you , it was impossible 
to get ligation succesfull with fragments purified by 
GeanClean after enzymic digestion, I dont no why.
The only thing worked at the end in my hands is the "old" 
purification by electroelution followed by phenol-chloroform 
purification and alcohol precipitation.
Cheers
Anis





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