DNA Quantitation-Quick and Dirty

David Barrass Barrass at pplros.demon.co.uk
Wed Aug 21 10:51:12 EST 1996


I need a rapid method of determining DNA concentration of a large number
of
samples on a routine basis.  The values will be in the range 100ng/ml to
1ug/ml
but the actual concentration is only required roughly (a factor of about
2).
Protein and RNA contamination is a problem in these samples.
 A260/A280 is affected by contaminants and slow
 Fluorometry (as we do it, Hoefer TKO 100) is way too slow0
 
 Any suggestions on this?



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