blunt end ligation problems

Vladimir Svetlov vvsvetlov at utmem1.utmem.edu
Wed Aug 14 19:22:44 EST 1996


In article <ahouse-1208961639130001 at 129.64.32.172>,
ahouse at hydra.rose.brandeis.edu (Jeremy C. Ahouse) wrote:

> Hello group,
>    we are having a hard time with blunt end ligation.


I need to say that I am not emotionally attached to blunt-end ligation and
if possible try to live w/out it. So far two amends to standard ligation
made biggest improvement in efficiency of ligation - using high
concentration T4 ligase from Pharmacia and/or addition of RNA ligase (the
latter increased efficiency of ligation appr. 10 fold). Both are quite
expensive (compared to Pap & Mom T4 ligases) but also produce results.
Regards,
VS



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