Antibody contamination during immunoaffinity purification
dykim at kaist.ac.kr
Tue Aug 20 02:12:08 EST 1996
Hello, I need some help
I purified the connexin 43 using polyclonal antibodies
which were crosslinked to Sepharose CN4B. My problem is that the eluted
connexin 43 contains the antibodies. I suspect that this antibodies were
dissociated during the acidic elution steps(pH 2.4). In the case of using
monoclonal antibody, no antibody contaminat were shown. I want to
prevent the dissociation of antibodies during elution steps.
Is there anyone to meet similiar problems like me? Any suggestions will
KAIST, Daejon Korea. Dooyeon Kim ......................
dykim at bioneer.kaist.ac.kr
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