Antibody contamination during immunoaffinity purification

[Dooyeon Kim]

Hello, I need some help  

I purified the connexin 43 using polyclonal antibodies

which were crosslinked to Sepharose CN4B. My problem is that the eluted

connexin 43 contains the antibodies. I suspect that this antibodies were

dissociated during the acidic elution steps(pH 2.4). In the case of using

monoclonal antibody, no antibody contaminat were shown. I want to 

 prevent the dissociation of antibodies during elution steps. 

Is there anyone to meet similiar problems like me?  Any suggestions will

be appriciated.              


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KAIST, Daejon Korea.                     Dooyeon Kim ......................
                                         dykim at bioneer.kaist.ac.kr
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