Glycogen for co-precipitating DNA

Paul N Hengen pnh at ncifcrf.gov
Tue Aug 20 14:33:08 EST 1996


jwobrien (jwobrien at students.wisc.edu) wrote:

> Does anyone have references for co-precipitating DNA with glycogen?
> Any protocols?  i.e. what concnetration is best.
> 
> BTW does anyone know if glycogen is what is in pellet paint??
> 
> John

Novagen say it is a glycogen-based polymer with a rhodamine-like
attachment.

@article{Helms1985,
author = "C. Helms
     and M. Y. Graham
     and J. E. Dutchik
     and M. V. Olson",
title = "A new method for purifying lambda
{DNA} from phage lysates",
journal = "DNA",
volume = "4",
number = "1",
pages = "39-49",
comment = "mussel glycogen as carrier",
year = "1985"}

@article{Tracy1981,
author = "S. Tracy",
title = "Improved rapid methodology for the
isolation of nucleic acids from agarose gels",
journal = "Preparative Biochem.",
volume = "11",
number = "3",
pages = "251-268",
comment = "oyster glycogen used as carrier",
year = "1981"}

@article{Hengen1996Junetibs,
author = "P. N. Hengen",
title = "Methods and reagents - Carriers for
precipitating nucleic acids",
journal = "Trends in Biochemical Sciences",
volume = "21",
number = "6",
pages = "224-225",
month = "jun",
year = "1996"}

--
*******************************************************************************
* Paul N. Hengen, Ph.D.                           /--------------------------/*
* National Cancer Institute                       |Internet: pnh at ncifcrf.gov |*
* Laboratory of Mathematical Biology              |   Phone: (301) 846-5581  |*
* Frederick Cancer Research and Development Center|     FAX: (301) 846-5598  |*
* Frederick, Maryland 21702-1201 USA              /--------------------------/*
* - - -  Methods FAQ list -> ftp://ftp.ncifcrf.gov/pub/methods/FAQlist - - -  *
* -  TIBS column archive -> http://www-lmmb.ncifcrf.gov/~pnh/readme.html - -  *
* - The BEST Molecular Biology HomePage -> http://www-lmmb.ncifcrf.gov/~pnh/  *
*******************************************************************************



More information about the Methods mailing list