Sections not sticking to slides
jhmorris at utmdacc.mda.uth.tmc.edu
Thu Aug 22 16:11:02 EST 1996
> I think people in our lab (who do in situs) use amino-silane-compounds
> with which they treat their slides instead of using Poly-Lysine. To my
> knowledge this treatment results in covalent attachment of the
> aminosilane to the glass surface. One seems to get a very high density
> of positive charges on the surface of the slide. Maybe this could do
> the trick...
> Tell me if you are interested and I'll try to find some suitable
> protocols for you.
I use these slides (APES-treated) for in situs also. You can buy them
already treated from a company called Histology Control Systems, catalog #
I've never had a problem with sections coming off. Maybe it's the slides.
(Gotta be the shoes)
--Julia Hsi Morris
jhmorris at utmdacc.mda.uth.tmc.edu or zzhao at utmdacc.mda.uth.tmc.edu
Dept. of Neuro-Oncology, Box 316
University of Texas M.D. Anderson Cancer Center
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