re-using electroporation cuvettes
css at box-c.nih.gov
Fri Aug 23 09:57:59 EST 1996
In article <spenneb-2308961620170001 at vgsec.uni-muenster.de>, "Ralf
Spenneberg" <spenneb at uni-muenster.de> wrote:
> Hi there!
> I was told that cuvettes used for the electroporation of E.coli could be
> reused. I was thinking that someone might have tried out different
> detergent for cleaning and has established a most effective method.
> Any help would be appreciated.
We use invitrogen cuvettes and after electroporating we rinse them two or
three times with water and then once with 70% EtOH. We then leave them
filled with 70% EtOH overnight. The following day way bake them for 20
minutes or so. Baking for longer tends to cause the cuvettes to warp or
crack. Hope this helps
> < /\~~~/\
> ^ ^ > <
> (_O_) > ^ ^ <
U > (_o_) <
More information about the Methods