FITC fading kinetics

Graham Atherton grggta at picr.cr.man.ac.uk
Fri Aug 23 10:07:35 EST 1996


Has anyone a feel for the rate at which FITC and similar fade when used for
immunohistochemical staining of Rat cells. I seem to get significant fade
over the first fews days and weeks - this means I cannot go back to reasses
slides a week old despite keeping them in Citiflour in the dark at 4oC. Any
way to minimise this problem?
Many Thanks
Graham Atherton
grggta at picr.cr.man.ac.uk



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