Eliminate light/heavy chain after IP??
dla at galactose.mc.duke.edu
Fri Aug 23 08:48:02 EST 1996
I'm doing some IP/Western assays. My protein comigrate exactly with the
light chain, so after IP and blotting the light chain mask the detection
of my protein. I've tryied Laemli buffer without DTT/betaMSH and even
though 95% of the IgG runs as a high MW, the remaining 5% still is enough
to interfere with detection. Does anyone know any protocol to get rid of
IgG after IPs?
Please, e-mail me to
altschul at med.unc.edu
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