Ligating small insert
rennie at bldghsc.lan1.umanitoba.ca
Fri Aug 23 15:08:16 EST 1996
In article <321A3780.738E at ea.oac.uci.edu>, "Minh Q. Nguyen"
<mqn1 at ea.oac.uci.edu> wrote:
> Has anyone tried ligating a very small insert (~50 bp) to your typical
> vector (e.g. Bluescript)? If so, what is the optimal ratio of insert
> to vector and how efficient is the ligation? Any suggestion would be
> appreciated. Thanks.
Yes I have done such a ligation. I believe we went for the standard
200ng of vector and tried to get around 1 ug of the insert. It went just
about as well as any ligation. It was a blunt too so that may have caused
the efficiency to be slight lower.
Hope this helps
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