Ligating small insert

S.L. Rennie rennie at bldghsc.lan1.umanitoba.ca
Fri Aug 23 15:08:16 EST 1996


In article <321A3780.738E at ea.oac.uci.edu>, "Minh Q. Nguyen"
<mqn1 at ea.oac.uci.edu> wrote:

> Hi,
> 
> Has anyone tried ligating a very small insert (~50 bp) to your typical 
> vector (e.g. Bluescript)?  If so, what is the optimal ratio of insert 
> to vector and how efficient is the ligation?  Any suggestion would be 
> appreciated.  Thanks.
> 
> mqn


HI
   Yes I have done such a ligation.  I believe we went for the standard
200ng of vector and tried to get around 1 ug of the insert.  It went just
about as well as any ligation.  It was a blunt too so that may have caused
the efficiency to be slight lower.

Hope this helps
Sharon

-- 





More information about the Methods mailing list