LiCl in DNA prep
dspinella at chugaibio.com
Fri Aug 23 15:57:30 EST 1996
LiCl can be used to remove RNA from DNA preps. Large RNAs (rRNA,
mRNA, etc.) are insoluble in solutions with high salt concentrations
(and its easiest to achieve high concentration with LiCl because it is
more soluble than NaCl), while DNA and small RNAs remain soluble.
Typically one must adjust the LiCl concentration to 4M to achieve the
differential "salting out" of RNA. The more common use of LiCl is to
purify the RNA from contaminating DNA, but the procedure can be used
in either direction. Hope this helps.
D.G. Spinella, Ph.D.
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