LiCl in DNA prep

Taek H. You tyou at postbox.acs.ohio-state.edu
Sat Aug 24 01:33:59 EST 1996


In article <321DDA06.38BE at gnv.ifas.ufl.edu> Rodney Earl Pettway <REPE at gnv.ifas.ufl.edu> writes:
>Path:
>magnus.acs.ohio-state.edu!csn!nntp-xfer-1.csn.net!carbon!night.primate.wisc.edu!
>ames!enews.sgi.com!news.sgi.com!spool.mu.edu!usenet.eel.ufl.edu!usenet.nerdc.ufl
>.edu!usenet.ufl.edu!usenet
>From: Rodney Earl Pettway <REPE at gnv.ifas.ufl.edu>
>Newsgroups: bionet.molbio.methds-reagnts
>Subject: LiCl in DNA prep
>Date: Fri, 23 Aug 1996 16:19:18 +0000
>Organization: University of Florida
>Lines: 5
>Message-ID: <321DDA06.38BE at gnv.ifas.ufl.edu>
>NNTP-Posting-Host: rthomas.ifas.ufl.edu
>Mime-Version: 1.0
>Content-Type: text/plain; charset=us-ascii
>Content-Transfer-Encoding: 7bit
>X-Mailer: Mozilla 2.01 (Macintosh; I; PPC)


>Can anyone answer this.  What role does LiCl play in DNA extraction 
>protocols?  This person in our lab uses it in their DNA extraction 
>protocol and I can not figure out why and they don't know either.

>Rodney Earl Pettway

LiCl precipitate RNAs at proper concentration.
If the step is not for this, it may have another role which I am not aware of.




More information about the Methods mailing list