CAT gene -- fluorescent substrates?
Gene Huh
gshuh at garnet.berkeley.edu
Fri Aug 23 19:14:23 EST 1996
Hello fellow netters,
I have a question for those who work with the chloramphenicol
acetyltransferase gene. I was wondering if a substrate for CAT exists
that
(i) is fluorescent and
(ii) has a reaction product that is retained within a live cell.
Such a substrate night work in the same way that FDG
(fluorescein-D-galactopyranoside) works for live staining of LacZ-positive
cells.
Thanks in advance,
Dr. Gene S. Huh
Dept. of Molecular and Cell Biology
University of California at Berkeley
gshuh at garnet.berkeley.edu
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