CAT gene -- fluorescent substrates?

Gene Huh gshuh at garnet.berkeley.edu
Fri Aug 23 19:14:23 EST 1996


Hello fellow netters,
I have a question for those who work with the chloramphenicol
acetyltransferase gene.  I was wondering if a substrate for CAT exists
that 

   (i)  is fluorescent and
   (ii) has a reaction product that is retained within a live cell.

Such a substrate night work in the same way that FDG
(fluorescein-D-galactopyranoside) works for live staining of LacZ-positive
cells.

Thanks in advance,
Dr. Gene S. Huh
Dept. of Molecular and Cell Biology
University of California at Berkeley
gshuh at garnet.berkeley.edu



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