Sequencing directly from gel slices ?

Bryan L. Ford fordb at
Fri Aug 23 18:22:04 EST 1996

Does anyone have a reliable method for directly cycle sequencing using an excised
agarose gel band as template, without further purification? My typical template
would be a strong PCR product in the range of 500 bp. I would use a nested
sequencing primer. I have a vague recollection of some protocol using LMP agarose
dirctly in this regard, but cannot presently locate any references. Thanks for any
assistance you may give me.

Bryan L. Ford
Marine/Freshwater Biomedical Sciences Center
Oregon State University
Wiegand Hall 228
Corvallis, OR, 97331-6603
Ph: (541) 737-6515 Fax: (541) 737-1877

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