re-using electroporation cuvettes

Ashok Aiyar aiyar at ebv.oncology.wisc.edu
Fri Aug 23 13:12:12 EST 1996


On Fri, 23 Aug 1996 16:20:17 +0100, Ralf Spenneberg <spenneb at uni-muenster.de> wrote:
>Hi there!
>
>I was told that cuvettes used for the electroporation of E.coli could be
>reused. I was thinking that someone might have tried out different
>detergent for cleaning and has established a most effective method.

I rinse the cuvettes (Biorad 0.2 cm and 0.1 cm)  with distilled 
de-ionized water thrice and with seventy percent ethanol twice.

They seem to work well enough for the transformations we do.

Ashok
-- 
Ashok Aiyar, Ph.D.                      
Department of Oncology            email: aiyar at ebv.oncology.wisc.edu
University of Wisconsin-Madison                  tel: (608) 262-6697




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