EMSA problems

Alexander Schmiechen alexander.schmiechen at student.uni-tuebingen.de
Fri Aug 23 11:23:23 EST 1996


Hi there!

We are having massive problems with "smiling" in our EMSA gels. We are
using a low-ionic strength running buffer (see Current Protocols). 

We have heard that omitting glycerol from the gels might help. Is this
true? Are there any other tricks?

We would appreciate any comments.

Dept. of Toxicology, University of Tuebingen, Germany




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