Help me! / Yeast transformation in Two-Hybrid screening

[Sourav Ghosh]

In article <DwHL7w.Iz7 at cf.ac.uk>, Joe Boutell <boutell at cf.ac.uk> wrote:

> naganot at bri.niigata-u.ac.jp (Takashi Nagano) wrote:
>Hi,
Gietz's lab http address is:
www.umanitoba.ca/faculties/medicine/human-genetics/gietz/Trafo.html
Their protocol works better than any other methods I have tried incl.
electroporation.  However, effiencies vary with strains and with
transforming plasmid quality and I think size too.  Working in small
batches helps too.
Ciao,
   Sourav