Help me! / Yeast transformation in Two-Hybrid screening

Sourav Ghosh sghosh at utmem1.utmem.edu
Sat Aug 24 14:58:29 EST 1996


In article <DwHL7w.Iz7 at cf.ac.uk>, Joe Boutell <boutell at cf.ac.uk> wrote:

> naganot at bri.niigata-u.ac.jp (Takashi Nagano) wrote:
>Hi,
Gietz's lab http address is:
www.umanitoba.ca/faculties/medicine/human-genetics/gietz/Trafo.html
Their protocol works better than any other methods I have tried incl.
electroporation.  However, effiencies vary with strains and with
transforming plasmid quality and I think size too.  Working in small
batches helps too.
Ciao,
   Sourav



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