Biotin Pcr primers and anealing temperature

[Timothy R Jackson]

if you think that it's a problem with changing annealing temps, why don't 
you try a touchdown pcr program.  check out BioTechniques 20:478-485 
(mar.96) for an example program.  we use a touchdown program of our own 
for a whole mess of 33p 5' end-labelled microsatellite primers, no matter 
the annealing temperature, and it works great!

Tim Jackson
Dept. of Zoology
University of Guelph
Guelph, Ontario
Canada