LambdaGEM 11 Xho gLibrary

Carsten Hoege c.hoege at bham.ac.uk
Sun Aug 25 07:25:29 EST 1996


In article <Pine.SUN.3.92.960823082840.22270C-100000 at molecule.bio.uts.edu.au>, michelle at MOLECULE.BIO.UTS.EDU.AU (Michelle Gleeson) says:
>
>Hi all,
>
>I'd like to hear from anyone who has sucessfully used Promega's LambdaGEM
>11 Xho1 half site arms for constructing a genomic library - hints,
>tips, how much gDNA used, what to avoid etc.
>Thanks in advance,
>Michelle

Hi Michelle!

If you need a large primary library, the half site arms are not the best choice, unless you size fractionate your genomic DNA. Small fragments ligate to the vector but do not yield viable phage!

Carsten
>michelle.gleeson at uts.edu.au
>Molecular Parasitology Unit
>University of Technology, Sydney
>



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