RNA sizing against DNA markers

Bernard Murray bernard at elsie.nci.nih.gov
Sun Aug 25 13:56:27 EST 1996


In article <4vq45a$276 at mserv1.dl.ac.uk>, NIBHARLO at acadamh.ucd.ie says...
>
>Hello everybody!
>A question on behalf of a colleague. She has some photos of old RNA 
>gels (agarose) with DNA markers on. What allowances does she make for 
>sizing the RNA against these markers? Please dont suggest she runs 
>them again, shes writing up and not in a position to do so.
>Thanks if you can help
>Gill

How about just running one gel.  This would of course have the same
DNA standards that she was using and some "real" RNA standards
(begged, stolen or borrowed) to allow you to calibrate one with the
other.  Alternatively, if the gels contains total RNA, you can generate
a rough (2 point) calibration using the 18S and 28S rRNA bands
as the equivalent size in bases is generally known for most species
(there may also be enough carry-over to see these if the RNA is
poly(A+) from total RNA - if its from in vitro transcription etc.
then you have a problem).
	Sla/n agus go n-e/iri/ an t-a/dh leat!
		Bernard

Bernard Murray, Ph.D.
bernard at elsie.nci.nih.gov  (National Cancer Institute, NIH, Bethesda MD, USA)




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