AmpliTaq Gold vs AmpliTaq: where is the magic ?
sgriffit at rpc.unb.ca
Sun Aug 25 12:06:15 EST 1996
In article <1996Aug19.162654.3778 at alw.nih.gov>,
bernard at elsie.nci.nih.gov (Bernard Murray) wrote:
>In article <4v4sou$sv7 at erika.cica.es>, bb1dopeg at lucano.uco.es says...
>>Anybody knows what is the key difference between AmpliTaq Gold vs
>>AmpliTaq at the molecular/reagent composition level? Apparently they
>>are both the same MW enzyme. I wonder if Gold is not something similar
>>to the Clontech Taq plus thermolabile antibody...
>No. I talked to one of their reps about this and she stated she was
>not allowed to say exactly what it was but it was definitely not an
>inhibitory antibody. I asked if it was "something like a heat labile
>blocking group, maybe for a cysteinyly thiol" and she said that it was
>along those lines. This would fit in with the lack of effect on its
> Does anyone know if the activity of Taq polymerase can be
>modulated within the bacterium? - maybe phosphorylation.
> Anyway, the reps feel that we don't have to know the nature
>of the control in the Gold enzyme as they claim that whatever the
>block is it will have no effect at all on downstream usage. So, if
>anyone can show a stituation where AmpliTaq works but AmpliTaq Gold
>does not I'd be interested to hear about it (and so would the
>manufacturers, I'm sure).
>Bernard Murray, Ph.D.
>bernard at elsie.nci.nih.gov (National Cancer Institute, NIH, Bethesda MD,
> After 5 years fiddling around with TaqStart antibodies TMAC, acetamide
SSB proteins etc etc,the promises of amplitaq gold were extremely
exciting.Unfortunately the claims that it can be substituted with little
if any changes to existing programs do not appear to apply to our many
different applications including multiplexing of dinucleotide repeats and
diagnostic PCR to detect bacteria in tissue lysate: in the latter
application the amplification was reminiscent of our titrations of
TMAC--wacking great bands in some positive samples but nothing at all in
others.In this instance Amplitaq LD and Clontech's TaqStart antibody beat
it hands down in reproducibility and sensitivity. I'm following all the
instructions dutifully, but I may be missing the boat on some little
Best Wishes STEVEg
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