Southern Blotting of Pulse Field Gels.... HELP!!?
dellaire at ODYSSEE.NET
Sun Aug 25 23:07:28 EST 1996
I am going to be blotting DNA resolved by pulse field electrophoresis
and I am worried about what protocol to use...
Should I use a regular southern protocol as for genomic DNA? Or are
there some modifications I should employ? What is a good membrane for
this kind of transfer?
I would like to hear about your experience with this technique...
Any and all suggestions needed and appreciated,
Div. of Exp. Medicine
dellaire at odyssee.net
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