Southern Blotting of Pulse Field Gels.... HELP!!?

Graham Dellaire dellaire at ODYSSEE.NET
Sun Aug 25 23:07:28 EST 1996

Hello all,

I am going to be blotting DNA resolved by pulse field electrophoresis 
and I am worried about what protocol to use...

Should I use a regular southern protocol as for genomic DNA?  Or are 
there some modifications  I should employ?  What is a good membrane for 
this kind of transfer?  

I would like to hear about your experience with this technique...

Any and all suggestions needed and appreciated,

G. Dellaire
Div. of Exp. Medicine
McGill University
dellaire at

More information about the Methods mailing list