Southern Blotting of Pulse Field Gels.... HELP!!?

Graham Dellaire dellaire at ODYSSEE.NET
Sun Aug 25 23:07:28 EST 1996


Hello all,

I am going to be blotting DNA resolved by pulse field electrophoresis 
and I am worried about what protocol to use...

Should I use a regular southern protocol as for genomic DNA?  Or are 
there some modifications  I should employ?  What is a good membrane for 
this kind of transfer?  

I would like to hear about your experience with this technique...

Any and all suggestions needed and appreciated,


G. Dellaire
Div. of Exp. Medicine
McGill University
 
dellaire at odyssee.net



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