LiCl in DNA prep

H. ROYCHOWDHURY hroychow at NMSU.EDU
Mon Aug 26 10:35:53 EST 1996


On Fri, 23 Aug 1996, Rodney Earl Pettway wrote:

> Can anyone answer this.  What role does LiCl play in DNA extraction 
> protocols?  This person in our lab uses it in their DNA extraction 
> protocol and I can not figure out why and they don't know either.
> 
> Rodney Earl Pettway
> 
> 
At 2M, LiCl precipitates most of the RNA in the prep. The smaller pieces 
of RNA, however, is difficult to precipitate in this way and a subsequent 
RNase treatment is necessary. At this high salt concentration, some of 
the proteins also precipitate along with RNA.

			>>>>>>>>>>>>>>>>>>>>>>>>>>>
			  Dr. Hiranya S. Roychowdhury
   			  Plant Genetic Engineering Lab.
			  Box 3GL, NM State Univ.
			  Las Cruces, NM 88003
			  Phone: (505) 646-5785
			  hroychow at nmsu.edu
			<<<<<<<<<<<<<<<<<<<<<<<<<<<




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