pGex expression

Ron Tate rtate at bmb-fs1.biochem.okstate.edu
Mon Aug 26 16:43:05 EST 1996


Giang Tran wrote:
> 
> I am trying to express some proteins in the pGex system. Can anyone tell
> me what would be the most suitable buffer to sonicate the cells in?
> 
> Thanks

I have recently been given a pGEX expression clone and the protocol 
uses PBS pH 7.2 with 1.0% Triton X-100, 5mM EDTA, Leupeptin, Aprotinin
and PMSF added. It seems to work well.
Happy Hunting
-- 
>>>>>>--------------------------->
Ron
Tate                                                                   
Lab of Franklin Leach
Dept. of Biochem. & Molecular Biology
Oklahoma State University 
rtate at bmb-fs1.biochem.okstate.edu
(405) 744-9326
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