DNA Quantitation-Quick and Dirty

Karl Voss karl at hobbes.chem.ualberta.ca
Mon Aug 26 17:02:29 EST 1996

David Barrass (Barrass at pplros.demon.co.uk) wrote:
> I need a rapid method of determining DNA concentration of a large number
> of
> samples on a routine basis.  The values will be in the range 100ng/ml to
> 1ug/ml
> but the actual concentration is only required roughly (a factor of about
> 2).
> Protein and RNA contamination is a problem in these samples.
>  A260/A280 is affected by contaminants and slow
>  Fluorometry (as we do it, Hoefer TKO 100) is way too slow0
>  Any suggestions on this?

We do all our quantitations by ethidium bromide fluorescence in a Turner
flourmeter.  This works very well and one can measure 4 or 5 samples per
minute.  If you have a lot of cash laying arround you could go for a 
microtitre plate based fluormeter.  Protein and RNA contaimation is a 
problem but there are ways of dealing with that.  

I know that this does not help much but I don't think there is a much
faster method of quantitation then fluorescence.  

Good luck.



If it ain't broke it doesn't have enough
features yet

Karl Voss
Department of Chemistry
University of Alberta
Edmonton, Alberta, Canada
T6G 2G2

karl.voss at ualberta.ca
phone 403-492-0222
fax   403-492-8231

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