antibiotics for LBA4404

Byeong-ha Lee byeongha at PLAZA.SNU.AC.KR
Mon Aug 26 20:14:38 EST 1996


Dear Peijun;
A. tumefaciens strain LBA4404 is resistant to 'streptomycin'.
and pBI121 has kanamycin-resistant gene (NPT II).
I usually add both antibiotics to media (YEP) at 50ug/mL.
You can apply only streptomycin to nontransformed LBA4404.
and both to transformants.
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    Byeong-ha Lee                 You Can Reach Me Through...
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On 26 Aug 1996, pzhang wrote:

> Hi, there.
> I have a simple question, but I realy need somebody help me out.
> 
> I try to transform Agrobactrium tumefaciens Strain LBA 4404 with binary plasmid pBI121. I am not sure what kinds of antibiotics I should used in medium or plate for growing non-transformed and transformed LBA 4404.
> 
> I appreciate any advises and suggestions.
> -- 
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