Cycle sequence results?

Eric W. Linton elinton at
Mon Aug 26 18:48:21 EST 1996

         We have been cycle sequencing for over 2 years now using 33P.
Recently using template and primers that have given good readable sequence
has resulted in all primers giving high backgroud with any visible
sequence in all lanes looking the same.
         This has happend very rarly before, does anyone know what causes
this and what the sequence that we can read is, since it is not our
organisms sequence.



Eric Linton
Rutgers, The State University of New Jersey
Cell and Developmental Biology
E-mail: elinton at
web page:
snail mail:
        Rutgers/Nelson Labs C-109
        P O Box 1059
        Piscataway, NJ 08855-1059

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