cleavage of MBP fusion proteins

Guriq Basi GBasi at SLIP.NET
Mon Aug 26 16:24:39 EST 1996


We have repeatedly attempted to cleave the maltose binding protein from 
our fusion proteins of interest using factor XA and found that the 
cleaved MBP no longer binds to amylose.  Has anyone out there 
encountered this same problem and if so, how were you able to overcome 
it?  (P.S.  The fusion protein itself is able to bind amylose resin 
before cleavage, just not after cleavage with factor XA).
please reply to gbasi at slip.net,  thanks for your help.



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