cleavage of MBP fusion proteins
GBasi at SLIP.NET
Mon Aug 26 16:24:39 EST 1996
We have repeatedly attempted to cleave the maltose binding protein from
our fusion proteins of interest using factor XA and found that the
cleaved MBP no longer binds to amylose. Has anyone out there
encountered this same problem and if so, how were you able to overcome
it? (P.S. The fusion protein itself is able to bind amylose resin
before cleavage, just not after cleavage with factor XA).
please reply to gbasi at slip.net, thanks for your help.
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