ligation-mediated PCR

H YANG hyang at UVic.CA
Mon Aug 26 12:36:59 EST 1996


I used T7 polymerase and Sequenase 2.0 in LMPCR for first strand
extension. pUC 18 after UV-exposure and piperidine cleavage works as
template.  But I can't get any strand -extension.  Does anyone know what
may be the reason?


Haiyan




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