DNA size markers

[Dr. Douglas A. Johnson]

We have been using purchased phiX174 digested with HaeIII as the low 
molecular weight size marker for our RAPD gels. The cost is mounting as 
we turn more and more to RAPD technology. Can anyone suggest an easily 
made DNA/restriction enzyme combination that could substitute for the 
commercial marker set? I would prefer a sequenced plasmid as plasmid 
isolation in our hands results in much higher yields that phage RF.

Thanks

Doug Johnson

djohnson at oreo.uottawa.ca